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Fig. 3. Inhibition of PNU-142586 uptake by PPIs in HEK-hOAT3 cells. HEK-hOAT3 cells were incubated at 37◦C for 5 min with 1 µM PNU-142586 (pH 7.4) in the absence or presence of various concentrations of lansoprazole (A), <t>rabeprazole</t> (B), omeprazole (C), and esomeprazole (D). Each point represents mean ± S.D. of three separate experiments using three monolayers. When the standard errors of the means are small, they are contained within the symbols. The apparent IC50 values were calculated by fitting the data to a sigmoidal dose-response regression curve.
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Fig. 3. Inhibition of PNU-142586 uptake by PPIs in HEK-hOAT3 cells. HEK-hOAT3 cells were incubated at 37◦C for 5 min with 1 µM PNU-142586 (pH 7.4) in the absence or presence of various concentrations of lansoprazole (A), <t>rabeprazole</t> (B), omeprazole (C), and esomeprazole (D). Each point represents mean ± S.D. of three separate experiments using three monolayers. When the standard errors of the means are small, they are contained within the symbols. The apparent IC50 values were calculated by fitting the data to a sigmoidal dose-response regression curve.
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Fig. 3. Inhibition of PNU-142586 uptake by PPIs in HEK-hOAT3 cells. HEK-hOAT3 cells were incubated at 37◦C for 5 min with 1 µM PNU-142586 (pH 7.4) in the absence or presence of various concentrations of lansoprazole (A), <t>rabeprazole</t> (B), omeprazole (C), and esomeprazole (D). Each point represents mean ± S.D. of three separate experiments using three monolayers. When the standard errors of the means are small, they are contained within the symbols. The apparent IC50 values were calculated by fitting the data to a sigmoidal dose-response regression curve.
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Fig. 3. Inhibition of PNU-142586 uptake by PPIs in HEK-hOAT3 cells. HEK-hOAT3 cells were incubated at 37◦C for 5 min with 1 µM PNU-142586 (pH 7.4) in the absence or presence of various concentrations of lansoprazole (A), <t>rabeprazole</t> (B), omeprazole (C), and esomeprazole (D). Each point represents mean ± S.D. of three separate experiments using three monolayers. When the standard errors of the means are small, they are contained within the symbols. The apparent IC50 values were calculated by fitting the data to a sigmoidal dose-response regression curve.
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Fig. 3. Inhibition of PNU-142586 uptake by PPIs in HEK-hOAT3 cells. HEK-hOAT3 cells were incubated at 37◦C for 5 min with 1 µM PNU-142586 (pH 7.4) in the absence or presence of various concentrations of lansoprazole (A), <t>rabeprazole</t> (B), omeprazole (C), and esomeprazole (D). Each point represents mean ± S.D. of three separate experiments using three monolayers. When the standard errors of the means are small, they are contained within the symbols. The apparent IC50 values were calculated by fitting the data to a sigmoidal dose-response regression curve.
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Fig. 3. Inhibition of PNU-142586 uptake by PPIs in HEK-hOAT3 cells. HEK-hOAT3 cells were incubated at 37◦C for 5 min with 1 µM PNU-142586 (pH 7.4) in the absence or presence of various concentrations of lansoprazole (A), <t>rabeprazole</t> (B), omeprazole (C), and esomeprazole (D). Each point represents mean ± S.D. of three separate experiments using three monolayers. When the standard errors of the means are small, they are contained within the symbols. The apparent IC50 values were calculated by fitting the data to a sigmoidal dose-response regression curve.
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Fig. 3. Inhibition of PNU-142586 uptake by PPIs in HEK-hOAT3 cells. HEK-hOAT3 cells were incubated at 37◦C for 5 min with 1 µM PNU-142586 (pH 7.4) in the absence or presence of various concentrations of lansoprazole (A), <t>rabeprazole</t> (B), omeprazole (C), and esomeprazole (D). Each point represents mean ± S.D. of three separate experiments using three monolayers. When the standard errors of the means are small, they are contained within the symbols. The apparent IC50 values were calculated by fitting the data to a sigmoidal dose-response regression curve.
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Fig. 3. Inhibition of PNU-142586 uptake by PPIs in HEK-hOAT3 cells. HEK-hOAT3 cells were incubated at 37◦C for 5 min with 1 µM PNU-142586 (pH 7.4) in the absence or presence of various concentrations of lansoprazole (A), <t>rabeprazole</t> (B), omeprazole (C), and esomeprazole (D). Each point represents mean ± S.D. of three separate experiments using three monolayers. When the standard errors of the means are small, they are contained within the symbols. The apparent IC50 values were calculated by fitting the data to a sigmoidal dose-response regression curve.
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Fig. 3. Inhibition of PNU-142586 uptake by PPIs in HEK-hOAT3 cells. HEK-hOAT3 cells were incubated at 37◦C for 5 min with 1 µM PNU-142586 (pH 7.4) in the absence or presence of various concentrations of lansoprazole (A), <t>rabeprazole</t> (B), omeprazole (C), and esomeprazole (D). Each point represents mean ± S.D. of three separate experiments using three monolayers. When the standard errors of the means are small, they are contained within the symbols. The apparent IC50 values were calculated by fitting the data to a sigmoidal dose-response regression curve.
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Fig. 3. Inhibition of PNU-142586 uptake by PPIs in HEK-hOAT3 cells. HEK-hOAT3 cells were incubated at 37◦C for 5 min with 1 µM PNU-142586 (pH 7.4) in the absence or presence of various concentrations of lansoprazole (A), <t>rabeprazole</t> (B), omeprazole (C), and esomeprazole (D). Each point represents mean ± S.D. of three separate experiments using three monolayers. When the standard errors of the means are small, they are contained within the symbols. The apparent IC50 values were calculated by fitting the data to a sigmoidal dose-response regression curve.
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Fig. 3. Inhibition of PNU-142586 uptake by PPIs in HEK-hOAT3 cells. HEK-hOAT3 cells were incubated at 37◦C for 5 min with 1 µM PNU-142586 (pH 7.4) in the absence or presence of various concentrations of lansoprazole (A), rabeprazole (B), omeprazole (C), and esomeprazole (D). Each point represents mean ± S.D. of three separate experiments using three monolayers. When the standard errors of the means are small, they are contained within the symbols. The apparent IC50 values were calculated by fitting the data to a sigmoidal dose-response regression curve.

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Contribution of human organic anion transporter 3-mediated transport of a major linezolid metabolite, PNU-142586, in linezolid-induced thrombocytopenia.

doi: 10.1016/j.biopha.2024.116801

Figure Lengend Snippet: Fig. 3. Inhibition of PNU-142586 uptake by PPIs in HEK-hOAT3 cells. HEK-hOAT3 cells were incubated at 37◦C for 5 min with 1 µM PNU-142586 (pH 7.4) in the absence or presence of various concentrations of lansoprazole (A), rabeprazole (B), omeprazole (C), and esomeprazole (D). Each point represents mean ± S.D. of three separate experiments using three monolayers. When the standard errors of the means are small, they are contained within the symbols. The apparent IC50 values were calculated by fitting the data to a sigmoidal dose-response regression curve.

Article Snippet: Linezolid and rabeprazole were sourced from LKT Laboratories, Inc. (St. Paul, MN).

Techniques: Inhibition, Incubation